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Furthermore, the increased IL-1β raised the percentage of intestinal neutrophils, which endued the zebrafish with A. The results suggested that 10 mmol/kg SB addition mirrored the protective effect of YC4W by increasing the production of IL-1β. To identify the causal relationship between butyrate and the higher pathogen resistance, different concentrations of sodium butyrate (SB) were supplemented. Higher butyrate content and enhanced expression of IL1β were subsequently found in YC4W. The results showed that compared with CON and YC1W, YC4W significantly increased the abundance of short-chain fatty acids (SCFAs) producing bacteria and elevated the gene expression of nlrp3. To determine the possible reason, intestinal microbiota analysis and RNAseq were conducted. hydrophila while 1- or 2-week treatment (YC1W or YC2W) did not. We found that 3- or 4-week YC administration (YC3W or YC4W) increased the resistance against A. A strain (YC) affiliated to Pediococcus pentosaceus, a commonly used probiotic, was isolated from fish gut and the potential role of YC against Aeromonas hydrophila was detected in zebrafish. Probiotic administration is a potential strategy against enteric pathogen infection in either clinical treatment or animal nutrition industry, but the administration duration of probiotics varied and the underlying mechanisms remain unclear.
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Finally, to provide even more complex functionalities to these non-spherical polymer particles, iron oxide nanocrystals were grown within the polymer matrix resulting in superparamagnetic particles.Chengjie Shan 1, Miao Li 1, Zhu Liu 1,2, Rong Xu 1, Fang Qiao 1, Zhen-Yu Du 1 and Mei-Ling Zhang 1* A variety of particles shapes can be obtained, ranging from dimpled spheres, flattened spheres and Janus particles by varying the swelling ratio, always with excellent monodispersity and reproducibility. Our results show that only when both MEHQ and O 2 are present during the course of the polymerization, the particles shape can be controlled, from spherical to asymmetrical. Monodisperse non cross-linked polystyrene particles, used as seeds, are activated by the addition of an initiator, which promotes their swelling ability, and then swollen with a monomers mixture (methyl methacrylate, glycidyl methacrylate and ethylene glycol dimethacrylate), before being polymerized in presence of both MEHQ and O 2.
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In this work we prove that monodisperse non-spherical polymer particles possessing asymmetric Janus structure can be easily produced by using an activated swelling method in combination with a control of the rate of free radical polymerization through the addition of the inhibitors 4-methoxyphenol (MEHQ) and O 2. The preparation of particles with non-spherical shapes is a challenging endeavor, often requiring a significant ingenuity, complex experimental procedures and difficulties to obtain reproducible results.